information regarding
to gain of the present study is The aim the intake of LH by normal follicular and luteal cells of buffalo
inactive smooth ovaries. reference to tissues from ovaries, with
Leutinizing hormone was prepared from pituitaries obtained from
for preparation as antigen
Then used female buffaloes. mature
of the specific rabbit antiserum which was used for biological assay, immunological assay and immunofluorescence localization of buffalo LH receptors in ovarian tissues.
The results obtained from this investigation revealed that: The intake of buffalo LH by follicles of different sizes showed a parallel increase during maturation. The results obtained by biological and immunological assay were identical. Immunofluorescent technique revealed high LH intake by granulosa and thecal cells of large follicles. The LH intake was high with mature CL as compared to old ones. LH intake by preantral follicles and inactive ovaries were less than those obtained by the large sized follicles. It was concluded that, as the follicle matures, the receptors of the follicle to LH are increased to augment their growth and response to LH to induced ovulation. The loss of receptors during luteinizing does not involve LH activity.