invstigate the toxic effect of The present study was carried out to
fish, Tilapia Nilotica, Labeo three species of nila copper sulphate on Nilotica; and Gambosia affinis. LCs of copper sulphate using tap water media having hardness of 44.5-54.3 mg/L as calcium carbonte were
31.9, 17.5 and 0.39 ppm in Tilapia Nilotica, Labeo Nilotica and Gambosia affinis, respectively. The use of distilled water media containing 3.2-4.3 mg/L Caco, revealed that LC of the same fish were 0.22, 0.205 and 0.84 ppm, respectively. The clinical signs were recorded.