Twenty-two isolates of infectious bovine Rhinotracheitis, virus, were isoalted from genetal and Respiratory tract, and conjunctiva ofcattle. These isolates were compared with reference strains of IBRV (Colorado, Paramon & Hungerian strains) using restriction endonucleuse enzymes, (Eco R,, Bg, Bam HI and Hind III). Our result revealed that six isoltes were related to the Colorado (type) while the Paramon, (16 isolates reference were detected. No isoalte was reiated to the Hungerian strain.