In this study, the vaccinal strain of LT-virus was inoculated in embryonated chicken eggs via chorio-allantoic membrane, allantoic sac and yolk sac routes. Results showed that the site of virus multipliction is CAM where the highet titres were obtained. In both CAM and AS. routes, the titre reached on the 5th day post inoculation in AAF and CAM suspensions for both routes were almost the same. Therefore for vaccine production the allantoic sac route may be chosen, the best day for virus harvest would be the 5th day Pol. In yolk sach route the virus reached the allantoic sac starting on the 3rd day Pol. and gave the lowest titer in the 5th days P...