Fourteen days old chickens (local breeds), were inoculated subcutaneously and intranasally with adeno-virus (CELO). Depression with temporary effect on general health was observed after inoculation using two routes, without reproducing the disease, no liver lesions were observed while reisolation of the virus was done. Concerning biochemical changes in the blood serum constituents of chickens infected with CELO virus, the concentration of glucose, cholesterol (mg/dl) and total proteins (gm/dl) were estimated in the serum of infected and control birds. A significant increase of both glucose and cholesterol levels was observed in the serum of chicken infected subcutaneously and intranasally by CELO virus. Total protein levels decreased in serum of infected bird than non infected ones.