SUMMARY Seventy air samples collected from poultry houses located at different localities in Monofia Governorate and examined bacteriologically. It has been found that the mean value of total viable count, Coliform count (MPN) and faecal streptococci count (MPN) were 8X10, 141.4 and 82.2 bacteria per liter respectively. In addition, the obtained results revea led the isolation of various kinds of bacteria from examined air samples. The effect of each isoalte on the healthy condition of poultry was dis cussed.