Urea poisoning due to accidental access of large quantities of improper ly mixed concentrate of high urea content (4.2%) was diagnosed in 25 fattening buffalo calves. Three animals died and 2 were emergency slaughtered. Dullness, followed by muscular tremors, excessive salivation, rapid respiration, incoordination, moderate bloat, finally convulsions and opithotonus were the significant clinical signs observed. The charac teristic biochemical changes were increased blood urea (78.3+0.8 mg%), serum ammonia (1.2+0.03 mg) and serum glucose levels (95.6+0.2 mg %) .