Total protein and its fractions were investigated before and after vaccination of donkeys by Babesia equi antigen. The experiment was carried out on 8 donkeys aged 6 months and vaccin. Blood serum was obtained before and 10, 20, and 30 days post vaccination. The study revealed a highly significant increase in serum total proteins, alpha, beta, and gamma globulins which achieved a prominant elevation 10, 20, and 30 days post vaccination. Albumin absolute values were not significantly changed.