The present study was carried out on 12 clinically healthy adult dogs of both sexes. Cholecystotomy was performed in all dogs. These animals were divided into two equal groups. In the first group, the gallbladder wall was closed using N-Butyl-cyanoacrylate tissue adhesive while in the second group, dexon plus was used. The radiological and histopathological findings cleared that the use of tissue adhesive gave a good result in comparison to the use of dexon plus suture.