This study was carried out on 40 diseased buffaloe calves suffered from severe enteritis and 20 clinically healthy ones which served as control group. Enteropathogenic E. coli (0 : kon : 0... : Bici : 0 : B ) and Salmonella formed the main bacterial causative agents, they were isolated and identified from 80% and 20% of diseased rectal swab samples respectively. Pseudomonas aeruginosa, Shigella, and Candida albicans were also found in form of mixed infection with enterotoxigenic E.col. and Salmonella. They were isolated from 15%, 5%, and 20% of diseased rectal swab samples respectively. Antibiogram for the bacterial isolates revealed the best sensitive antibiotics were amikain, chloramphenicol, garamycin, netilimi cin, rimactan, and tobramycin. In diseased cases blood sera biochemical analysis showed highly significant (p < 0.01) decrease in serum glucose level with significant (p < 0.05) increase in total proteins concentration.