The use of GnRH-analogue (Fertagyl) for treatment of ovarian inactivity was tried in 5 Jersey COWS and 5 bufflo-cows having long acyclic post-partum period and with no structures in their gonads. Each Cow was given 250 ug of GnRH analogue and each
buffalo-cow was given 500 ug of the drug, as a -- single dose, by the intramuscular route of
injection, Signs of estrus were exhibited by 4 of the cows and 2 of the buffaloes, 72 hours after treatment. The remaining cow and one of the buffaloes came in heat 25 days after treatment but the other 2 buffalo-cows did not show signs of estrus, however, one of them had a palbable follicle in her ovaries. All cows who came in heat were naturally mated by fertile bulls and rectal examination, 6 weeks later, revealed that the 5 Jersey cows and 3 out of the 5 buffalo-cows were pregnant.