The present study was concerned with the detection of the most suitable condition for propagation and preservation of vaccinal strain of Reovirus. The most suitable route of inoculation for embryonated chicken eggs (ECE) was chorio-allantoic membrane (CAM) and allantoic cavity (AC) giving an infective titer 109 and 10' */ ml EID50 respectively. The best dilution of Reovirus used for egg inoculation was 10. Determination of the best time for harvesting the virus was the fourth day post inoculation. The suitable organ forvirus multiplication was embryo and mixture of embryo plus CAM and AAF giving a titer of 100*44 and EID50/ml respectively. Studies on the effect of using different stabilizers on the EID50 of lyophilized virus showed that skimmed milk gave the highest EID50 (10'.*/ml). Stability of Reovirus at 56°C for 8 hours.