A total of 289 serum samples were collected from calves and adult cows in Giza, Beny Sweif, Alexandria, Demiat and New Valley governorates. A Serum neutralization test (SNT) was conducted by microtitre on MA 104 cells, the results showed that rota virus infection exists in the country, where it reached 85.7% in calves, 50% in adult cows in Beny Sweif governorates, 54.5 in calves and 36.4% in cows in Demiat and reached 3.8% in calves and 3% in cows in Alexandria governorates while new valley governorate was clean. the result obtained by SN was reaffirmed by ELISA and indirect flourecent antibody technique.