SUMMARY A total of 662 samples including infertile eggs and dead inshell chicken embryos were collected from different balady hatcheries located in Dakahlia Governorate. The samples were examined bacteriologically for detection the actual bacterial causes of this hatching problems. The percentage of positive samples from infertile eggs and from dead in shell chickens embryos were 18.1 and 32.9% respectively E.coli isolates were the most prevalent organism isolated from infertile eggs with an incidence of
(26.4%) and from dead in shell embryos (21.9%). E.coli isolates were serologically identified Proteous, Pseudomonas, Staph aurecus, Klebsiella, Citrobacter and Enterobacter organisms were successfully isolated from infertile eggs with an incidence of 22.6%, 13.2%, 9.4%, 11.3%, 7.5%, and 7.5% respectively. The same organisms except Proteous were recovered from dead chicken embroys at rate of 10.4%, 17.1%, 11.6%, 11.6% and 5.5% respectively. Sulmonella was the lowest organism isolated from infertile eggs (1.8%) and from dead chicken embryos (7.3%). and they were serologically identified.