This work was carried on 12 clinically healthy buffalo calves (1-2 month old) devided into 2 eequal groups. The first group was the expermintal group where single intramuscular ingection of 20 mg oxytetracycline base /kg body weight was given to each animal. The scond group usded as control and was injected on normal physiological saline solution. In buffalo calves of the first group single intramuscular injection dose of oxytetracycline evoked a significant increase (Prtate aminotransferase (AST), Alkaline phosphatase (ALP) activities , total lipids, total cholesterol and serum total bilirubin levels. The p < strong>revious changes lasted for two weeks. On the similar grounds, oxytetracycline administration induced an elevation of serum arginase activity that lasted for one week In keeping with this line, oxytetracycline treated group displayed
a significant increase (P<0.05) of blood serum glucose level for one week pos treatment. Conversely, oxytetracycline injection produced a significant declin (P<0.05) of blood serum total protein and globulin levels for two weeks. Meanwhile oxytetracycline induced non significant changes in blood serum albumin levels in th treated buffalo calves