A total number of 262 donkeys, 17 horses and 14 mules were subjected to serological examination for brucellosis by tube agglutination (TAT), Rose Bengal (RBPT), buffered acidified plate (BAPA) and Coomb's tests. The percentages of seroreactors for TAT were 20.61%, 5.88% and 71.42% in donkeys, horses and mules respectively. That of RBPT were 12.97%, 5.88 and 71.42%, while the BAPA revealed 13.35%, 5.88% and 64.24%. The Coomb's test gave 13.35%, 0% and 14.28% in donkeys, horses and mules respectively. The present findings proved that TAT was the most sensitive one for the serological detection of brucellosis among equines.