SUMMARY Urethrotomy was performed on 12 male donkeys at the base of the penis in the perineal region. Suture closure of the urethra, subcuticular tissue and skin was performed in six donkeys, and the surgical site was allowed to heal by second intention in another six donkeys. Two donkeys from each group were euthanised at 2,4 and 12 weeks postoperatively. Urethral mucosal irregularity was seen on urethrograms performed in all opereted donkeys in different degrees, which were nearly similar in both groups. Urethral stricture was observed in the sutured group in severe and mild degrees at 2 and 4 weeks respectively, and in slight form at 12 week postoperatively while the stricture was present in the second intention group only at 4 weeks and decreased after that. As a conclusion, it should be emphasized that suturing of the urethrotomy wound in donkeys is preferable than keeping it without suture.