The brucella seroprevalence among 8774 cattle in four locations (Assiut, Manfalout, Sedfa and El-Ghanaem) belonging to Assiut governorate was estimated. All samples were screened by Rose Bengal plate antigen test (RBPT) and buffered acidified plate antigen test (BAPAT) and positive cases were confirmed by tube agglutination test (TAT) and rivanol tests. The results indicated that the prevalence proportions among different locations were 1.12%, 0.3%, 0.31% and 0.29% respectively. The brucella seroprevalence proportions in Assiut Governorate by different serodiagnostic tests were 0.89%, 0.87%, 0.6% and 0.57% respectively.