An investigation was carried out to find the seroprevalence of brucellosis in buffaloes in a large commercial dairy farm which had an alarming rate of abortions. Many abortions occurred in buffaloes at 7-9 months of pregnancy. The animals were subjected to various serological tests for brucellosis and it was found that no single test correctly identified all the animals. Aborted buffaloes showed titres against B.abortus antigen from 1/10 to 1/1/60. The overall incidence of brucellosis in females as revealed by blood tests was 19.9% and by milk ring test 12.3%. The incidence in examined males was 25%. The importance of carrying out milk ring tests for correct evaluation of brucellosis infection in buffaloes was stressed out.