During the study, 54 faecal samples from diarrhoeic and apparently healthy bufffalo and cow calves have been examined for detection of viruses associated with neonatal calf diarrhoea in the farm of the Faculty of Veterinary Medicine, Suez Canal University. The presence of Coronaries
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has been screened by using negative stain transmission electron microscopy, haemagglutination and virus isolation using Human Rectal Tumor cells (HRT-18 cells). The presence of other viruses (Root and Caliciviruses) has been detected only by electron microscopic examination. The electron microscopic examination revealed the presence of the characteristic morphology of Coronavirues particles in eight samples (14,7%), six of them were obtained from diarrhoeic calves and the remaining two from apparently healthy calves. Two samples revealed the presence of Coronavirus haemagglutinins (3,7%), one of them from a diarrhoeic calf, while the other from an apparently healthy calf. The electron microscopic examination detected also the presence of Rotavirus and Calicivirus, each in one sample. Isolation of Coronavirus particles on (HRT-18 cells) was not successful.