A serosurvey was done on 1267 equine serum samples collected in Egypt during 1994 and 1995 from previously vaccinated provinces, since 1994 (Aswan, Qena and Souhag) as well as from non-vaccinated provinces, since 1984 (including eighteen provinces in upper and lower Egypt). All sera were collected from non-vaccinated provinces, examined by the agar gel precipitation test (AGPT) gave negative results but previously vaccinated ones showed a low percentage of AHS specific immunoprecipitating antibodies (2.2%). Collected serum samples were subjected to complement
fixation test (CFT) and enzyme linked immunosorbent assay (ELISA) showed low percentages of African Horsesickness viral antibodies (AHSV antibodies) (1.3, 2.3 respectively) for non-vaccinated provinces and for those from previously vaccinated ones, it was 7.1%, 17.6% respectively. Serum neutralization indices (Ni) were also estimated for some selected serum samples. Very low percentages of neutralizing antibodies were detected in sera of equines from non-vaccinated provinces (not vaccinated since 1984) which ranged from 0.75% to 1.75% and significant percentages and titers were in sera from horses in previously vaccinated provinces (Vaccination was stopped by Jan. 1994) which were from 1% to 2.60%.