The use of Binary Ethylenimine as inactivant for Newcastle virus give a promising result as it has no effect on the antiginecity of the virus when used
or 0,03 M at the same time it had completely in a concentration of 0.01M reduced the virulence of the virus within 6 to 8 hours according to the concentation. This results were superior than the results obtained when using
INACTIVATION OF NEWCASTLE VACCINE
formaldehyde solution as inactivant in concentration of 0.1% or 0.2% which significantly reduced antigencity from 1024 to 32 HA titer. The experimental prepared batches of Binary in- activated vaccine as compared with formalin inactivated prepared vaccine by different adjuvents (oil-gel) proved that the BEI 0.01M with oil as adjuvent was the best as it gives the highest 10g2 HI titer (5.3) as well as the highest protection percentage 94%.