Eighty serum samples were screened from vaccinated chicken for infectious bursal disease virus (IBDV) antibody level after they had received a single dose and two doses of live attenuated IBDV vaccine (De). The first dose was given at 14 days of age via eye drop route while the second dose was given via drinking water oute. The antibody profile of primary and secondary were compared using agar gel precipitation test (AGPT), serum neutralization test (SNT) and enzyme linked immunosorbant assay (ELISA). The result revealed that the AGPT is useful for general screening purpose while the ELISA test is sensitive, specific, rapid and it consumes less time since large number of samples can be tested. The SNT appears to equal the
IMMUNE RESPONSE OF VACCINATED CHICKENS
ELISA test in indicating flock immunity, but the SNT is more expensive and less in time consuming.