In studying Listeria monocytogenes in rabbits, a total of "50" diseased as well as freshly dead animals suspected clinically and pathologically to suffer from the disease were examined. The affected rabbits were collected from different governmental and private farms at Assiut Governorate. They were subjected to post mortem as well as bacteriological examinations. Only nine positive cases of Listeria monocytogenes were isolated with an incidence of 18%. Identification of the causative organism was based on morphological,
biochemical and biological characters as well as histopathological lesions. Liver, uterus and brain showed the typical lesions of listeriosis in rabbit. The gravid and aborted uteri were severely affected than the non gravid one. The spleen showed severe heamorhage in the red pulp and exhaustion of the lymphocytic elementes. In vitro antibiotics sensitivity tests showed that the examined isolates were highly sensitive to gentamycin, tetracycline and spictinomycin