A total of 90 milk samples were collected from non-responding cow mastitic cases for antibiotics treatment from Giza and Kafr El-Sheikh Governorates. In addition, thirty samples were collected from apparently healthy cows.
The mycological examination of all samples revealed isolation of 23 (25.5%) strains of pathogenic yeasts and 8 (8.8%) strains of moulds from mastitic samples. These strains were identified as Candida albicans, C. parapsillosis, C. tropicalis, C. krusei, Trichosporon species, Torulopsis glabrata, Rhodotorula rubra, Aspergillus niger, A. fumigatus, Penicillium species, Mucor spp. and Absidia spp. Mycoplasma bovis was isolated from 42 (48.8%) while Mycoplasma bovigentalium was isolated from 15 (16.7%) of mastitic milk. The same results were detected by the indirect haemagglutination test. Mixed infection of fungi and mycoplasma were observed in 4 samples from Kafr El-Sheikh area. Chlorides, lactose, calcium, phosphorous, magnesium percentages, pH value and total cell count were examined.