A total number of 160 cervicovaginal mucus (CVM) samples were collected
non-pregnant cows in Fayoum Governorate. All samples were from
serologically to detect antibodies of Tritrichomonas fetus, examined
and cross reactivity between antibodies of the two Campylobacter fetus venereal diseases were tested by using microagglutination test and ELISA
test. In the present study, the results of microagglutination test for detection of antibodies of Tr. fetus and C. fetus were 8(5%) and 14 (8.75%) respectively in comparison to 19 (11.88%) and 33 (20.63%) by using ELISA test. The cross reactivity between Tr. fetus and C. fetus was positive in 6 (27.27%) cases by using microagglutination test but ELISA test was positive only one case (1.92%). This means that using of ELISA technique solves the problem of cross reactivity between Tr. fetus and C. fetus by demonstration of the specific IgG and IgA in the cervicovaginal mucus.