225 eggs were collected in summer months from Assiut city markets, different groceries, supermarkets and farmers' houses and examined for the presence of Enterobacteriaceae. The obtained results revealed that the mean value of total Enterobacteriaceae count / shell and count/ml of egg content samples were 4.1X10% and 2.7X102 respectively. The results also revealed that 71.11% and 44.44% of the examined egg shell and egg content samples were contaminated by Enterobacteriaceae respectively. The different isolates recovered
from the examined samples were identified as Escherichia coli (only 12 isolates could be typed serologically.), Proteus (vulgaris, rettergi and mirabilis); Enterobacter (aerogenes and cloacae); klebsiella (ozaenae and oxytoca); Citrobacter (diversus and Freundii); Serratia liquefaciens Providencia spp. and Salmonella typhimurium. The economic and public health importance of the isolated Enterobacteriaceae has been discussed and suggested measures for improving the quality of produced hen eggs are given.