A total of 80 rabbits 8-week old were used in this experiment and a cell-free culture filtrate (CCF) of P. multocida was used as a vaccine. The rabbits were divided into four groups. The first group was vaccinated intraocularly (eye dreop) (0.1 ml). Second group intranasally (0.1 ml) and the third group < /p>
was inoculated S/C with 0.5 ml. The fourth group was not vaccinated and served as a control. The second dose was given at 12 weeks of age. Sera were collected at 21 days after the first dose, 30 days after the second dose and 40, 60 and 90 days after challenge which was performed at 15 weeks of age. All sera were tested for anti P. multocida antibodies by using the enzyme linked immunosorbent assay (ELISA) and indirect haemagglutination test (IH). Challenge was conducted in vaccinated rabbits. Protection was superior in rabbits given CCP via intranasal route then the group vaccinated S/C came next. These results indicated that CCF could be considered as an effective immunogen when administered intranasally for protecting rabbits against Pasteurellosis and this was revealed by histopathological examination.