In this investigations 118 buffaloe calves ranging from 6 to 9 months old were carefully clinically and laboratory examined. Levels of some serum trace elements such as iron, total iron binding capacity copper and vitamin A, Beta-carotene and vitamin C were determined. In addition PCV and some biochemical values include serum total protein, albumin, creatinine and urea were also determined. Significant changes in these values in diarrhoeic buffalo calves due to bacterial infection were higher than those due to dietary causes which showed insignificant variations. It could be concluded that a substantial biochemical changes occured in diarrhoeic buffalo calves and may be due to bacterial causes and that must put in consider to increase renal function efficiency, repair the damaged intestinal epithelium and increase the body immune status during the infection alongside with the traditional electrolytes therapy