Local breed cattle in Behera province are investigated for Babesia bigemina infection. The incidence is found higher in Hoosh-Easa and Shobra- kheet followed by Damanhour and Etay El-Baroud respectively. Examination of   Giemsa stained blood films together with two serological tests [Capillary tube agglutination test (CAT) and Enzyme linked immunosorbent assay (ELISA)] are used in the diagnosis. In comparison, ELISA technique offers a number of advantages overall other tests; being more sensitive, requires a simple apparatus for large number of samples, can be automated (less subjected to operator error) and can also be adopted as a simple field screening test especially in intensive breeding programms and dairy farms.