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183164

DETECTION OF BRUCELLA ANTIBODIES IN COW MILK WITH DOT-ELISA KIT

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Last updated: 05 Jan 2025

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Abstract

Milk samples from 141 cows with gynaecological problems in a brucellosis endemic farm and from 50 apparently healthy cows of brucella free farm were tested with a newly developed field dot-ELISA kit. Serum samples of the same animals were checked for antibody titres with serum agglutination test (SAT), complement fixation test (CFT) and the plate enzyme linked immunosorbent assay (ELISA). The kit detected as negative all the 50 milk samples from brucellosis free farm. The corresponding serum samples of the cows were also found negative to the other serological tests. Of the 141 cows belonging to endemic farm, kit detected 95 as positive on milk testing, plate ELISA and SAT found 93 and 71, respectively, as positive, on sera testing. CFT was positive in 88 cases out of the 134 cows with 7 animals exhibiting anti-complementary activity. Detection of brucella antibodies in milk by the kit from individual cows appeared to correlate well with the serological results of the cows obtained by the plate ELISA and the CFT. The simple and convenient process of testing by the kit coupled to its high sensitivity and the specificity suggests its usefulness in routine field testing of milk samples from individual animals for the brucellosis disease surveillance programs.

DOI

10.21608/avmj.1998.183164

Keywords

Key Words: Brucellosis, Cow Milk, antibodies, ELISA

Authors

First Name

H.V.

Last Name

Batra

MiddleName

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Affiliation

Division of Microbiology, Defence Research and Development Establishment Gwalior 474002, India

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Orcid

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First Name

G.S.

Last Name

Agawal

MiddleName

-

Affiliation

Division of Microbiology, Defence Research and Development Establishment Gwalior 474002, India

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City

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Orcid

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First Name

Kiron

Last Name

Bala

MiddleName

-

Affiliation

Division of Microbiology, Defence Research and Development Establishment Gwalior 474002, India

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Orcid

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Volume

38.2

Article Issue

76

Related Issue

26324

Issue Date

1998-01-01

Receive Date

1997-12-03

Publish Date

1998-01-01

Page Start

242

Page End

250

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 38.2_Issue 76_Pages 242-250.pdf

PDF . 2.6MB

Link

https://avmj.journals.ekb.eg/article_183164.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=183164

Order

18

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

DETECTION OF BRUCELLA ANTIBODIES IN COW MILK WITH DOT-ELISA KIT

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Article

Created At

23 Jan 2023