Atotal of 200 freshly dead growing chickens collected from different governmental farms at Assiut Governorate. Only 16 positive cases of Pseudomonas (Ps) aeruginosa were isolated with an incidence of 8%. The experimental infection in 3-day old chicks by different routes of inoculation revealed that subcutaneous route was highly effective with mortality rate 95%, while oral route of infection was almost less (40% mortality). Reisolation of inoculated organism from dead chicks were conducted. In vitro antibiotics sensitivity tests showed that the examined isolates were highly sensitive to gentamycin, neomycin, danofloxacin, colistin, amikin and streptomycin.