A total of eighty fish samples (fifty samples of Oreochromis niloticus and thirty samples of Labeo niloticus) were collected from various fish examined All sampls were Assiut City. in located markets organoleptically and bacteriologically. All the examined samples were accepted organoleptically . A total of 345 and 178 bacterial strains were isolated from gills, intestine and muscles of O. niloticus and Labeo niloticus respectively. The average number of total aerobic colony count per gram muscles were 32.13 x10 / gram and 40.3 x 104 / gram for O. niloticus and Labeo niloticus respectively. All the bacterial isolates were identified morphologically, culturally and biochemically. No Salmonella species and E. coli could be recovered in the present investigation. Bacteriological examination of fishes must be associated with organoleptical examination to give accurate judgement. Correlation between the bacterial isolates and kind of fish as well as the public health significance of the isolated organisms were discussed.