A trial was made in Assiut to isolate Gram positive cocci (Staph and strept) from some poultry farms suffereing from septicaemia. Twenty four isolates of staaphylococci and forty streptococci isolates were recovered from diseasaed birds. In vitro sensitivity tests of staphylococcus and streptococcus isolates were carried out. Staphylococcus isolates were sensitive to Enrofloxacin and Kitamox while streptococcus isolates were sensitive to Garamycin, Chloramphenicol, Enrofloxacin and Ampicillin. The pathogenesity of Staph, aureus for 3 days chicks caused very virulent pictures and early deaths for chicks.