In a survey on fascioliasis in man and animals in Ismailia Province, the overall infestation rates were 107(7.8%), 205(14.99%) and 286(20.9%) out of 1368 animals as diagnosed by faecal, gall bladder examinations and Indirect haemagglutination test (IHAT), respectively. The Fasciola infestation rates as indicated by the previous tests respectively, were higher in sheep (12.4%, 22.9% and 26.7%) and goats (9.9%, 16.3% and 22.7%) followed by Cattle (5.3%, 10.9% and 18.8%). While the lowest prevalence rates were reported in buffaloes (3.7%, 9.1% and 15.2%). On the other hand, the human Fasciola infestation rates were 2 (0.4%) and 26 (4.9%) out of 533 human samples by stool examination and Indirect haemagglutination test, respectively. There was no significant difference in Fasciola infestation rate between male (3.9%) and female (5.9%). It was clearly that all ages were susceptible to Fasciola infestation. The serodiagnosis (IHAT) was more sensitive than faecal examination for detection of fascioliasis. These findings indicated that the infested animals are considered as a continuous contaminating source for the fresh water by Fasciola eggs. The infestation rate depends mainly on feeding habit of the people and their general hygienic measures. Faecal examination was not enough for diagnosis of this disease. On the other hand, the immunological diagnosis of fascioliasis based on detection of antibodies has proved to be a useful method to achieve this goal.