standard the chloramphinicol and oxytetracycline, Lincomycin, combination of penicillin G sodium streptomycin sulphate were evaluated for the control of Chlamydia psittaci in processed buffalo-semen. The chloramphinicol and oxytetracycline in different concentrations were effective, while lincomycin and the standard combination of penicillin and
streptomycin were not effective. On the other hand, the chloramphinicol and tetracycline were very toxic to sperm and its using as antibacterial agent in semen diluents should be contraindicated. In conclusion, Chlamydia psittaci could not be controlled in diluted semen samples by adding chemotheraputic drugs as the effective drugs on Chlamydia psittaci are very harmful to sperm picture. So, it is recommend that the semen samples must free from Chlamydia psittaci before using them in artificial insemination due to its hazard effect on animal reproduction.