A total of 140 random samples of fresh water and salted fishes including 115 Tilapia nilotica and 25 Hydrocynous forskallii salted fishes were collected from fish markets and from different Moloha's shops in Assiut
City. Bacteriological examination was carried out using both enrichment and selective media together with microaerophilic atmosphere for isolation of different biotypes of Campylobacter microorganisms. A total of 4 isolates of Campylobacter jejuni (3 of biotype 1 and 1 of biotype 2) and 3 isolates of Campylobacter coli were recovered from the examined Tilapia nilotica fishes. The salt contents and pH values of salted fishes were determined, while no Campylobacter organisms were recovered from salted fishs. The isolated organisms were tested for their sensitivity to 12 chemotherapeutic agents. Campylobacter jejuni/coli proved to be highly sensitive to Erythromycin and the Nalidixic acid with an activity of 100 %. The public health significance of these microorganisms, as well as recommended sanitary measures were also discussed.