Necrotic enteritis is well-defined as poultry disease which occures as a result of liberation of toxins from Clostridium perfringens type A, C, D, and G pathogenic strains. This study was performed to determine the prevalence rate of C.perfringens in broilers from different farms in Assiut Governorate and to examine the isolates for the existence of alpha, beta, epsilon, and netB gene. A sum of 100 intestinal specimens were compiled from diseased broiler chickens (3-6 W) which had clinical signs and post mortem lesions of Necrotic Enteritis and examinedby conventional and molecular methods. C.perfringens was isolated from 52% (52/100) of the bacteriologically examined flocks. Only ten isolates among suspected isolates were examined by using uniplex and multiplex PCR. The results reported 100% positivity for cpa and netB gene in the examined isolates and neither cpb nor etx gene were detected and proved that all isolates were Clostridium perfringens type G.