The present study was conducted on 156 cattle and buffaloes. The age of these animals between 6 months to 4years, they examined during the period from January 2018 to September 2019. These animals from different localities in Assiut, sohag and El-Menia governorates. The investigated animals characterized by sudden onset of fever in 25.64% (40 /156) ranged from 40˚ to 42˚C associated with severe congested mucous membranes, dry muzzle, foamy salivation, dullness, sickness, shivering and trembling, rapid shallow respiration in 19.23% (30/156) and ruminal stasis in 25% (39/156), enlargement of superficial lymph nodes. 7.05% (11/156) and 6.41% (10/156) of the examined cases showed drop in milk production and abortion, respectively. Little number of cases showed recumbency, subcutaneous emphysema and lameness respectively 1.28% (2/156), 0.64% (1/156) and 3.21% (5/156). The serological detection for ephemeral fever virus antigen was 9.52% (8 /84), and 4.76% (2 / 42) were positive for virus antibodies.  40/ 50 (80%) were positive for RT- PCR. The prevalence of infection was 17.95% (28/156) in Assiut governorate, 5.77% (9/ 156) in Sohag, 1.92% (3/ 156) in El-Menia. Frezian breeds had higher rate of infection 13.46% (21/156) than native breeds 6.41% (10/156) followed by buffaloes was 5.77% (9/156). The infection in females was 17.31% (27 / 156) but in males was 8.33% (13 / 156). The age group >2-4 years (13.45%) was more susceptible to virus infection than age group 6 months-2 years (12.5%). The infection rate was higher in hot months 19.23% (30/156) than non- hot months 6.41% (10/156).