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205248

ANTIFUNGAL DISINFECTANTS EFFICIENCY ON ASPERGILLUS STRAINS FROM CAMEL’S MILK AND DRINKING WATER: BIOLOGICAL DETOXIFICATION OF AFLATOXIN-M1

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Animal hygiene and Environmental pollution
Forensic medicine and toxicology
Laboratory investigation
Milk hygiene & milk product

Abstract

This study was conducted to assess the distribution of mold and mycotoxins in raw camel's milk and water samples and to evaluate the fungicidal activity of three disinfectants and the detoxification capabilities of LAB towards aflatoxin M1 in camel's milk. Molds were isolated from 56 and 64% of water and raw camel milk samples, respectively. Aspergillus spp < /em>. were the most abundant species isolated from water and raw camel milk samples with 10.9 and 11.6%, respectively, followed by Penicillium and Fusarium spp < /em>. The concentrations of aflatoxin-B1 (AFB1) and ochratoxin-A (OTA) in the examined water samples were 7.97 and 8.28 µg/l, respectively. Furthermore, the concentrations of aflatoxin-M1 (AFM1) and ochratoxin-A (OTA) in the examined raw camel milk samples were 1.89 and 1.69 µg/l, respectively. Quaternary ammonium/glutaraldehyde based disinfectant was the most efficient disinfectants against the isolated fungal species. On contaminated dairy surfaces; exposure of A. flavus to Quaternary ammonium/glutaraldehyde based disinfectant for shorter contact times less than one-hour exhibited reduced efficacy with log reduction not exceed 2.6 log, while for A. ochraceus, a minimum of 15 minutes' exposure time was required to induce good efficacy with 3.2 log reduction. Adjusting exposures time for one hour showed good efficacy for both A. flavus and A. ochraceus with 4.5 and 4.6 log reduction, respectively. The AFM1 concentration in prepared yoghurt from camel's milk was reduced to 24.6% after the first 5 days of incubation; and after incubation for 15 and 20 days, further reduction to 45.9 and 55.7% was observed, respectively.
Abbreviations: AFB1, Aflatoxin B1; AFM1, Aflatoxin M1; aflD and aflQ, Aflatoxin B1 biosynthesis genes: the structural genes aflD and aflQ; OTA, Ochratoxin A; CFU, Colony forming unit; FE, Fungicidal Effect; HPLC, High Performance Liquid Chromatography; LAB, Lactic acid bacteria; MPL, Maximum permissible limits.

DOI

10.21608/avmj.2021.205248

Keywords

camel milk, water, Aspergillus, Mycotoxins, LAB, fingicidal activity, disinfectant

Authors

First Name

HASSAN MAHMOUD

Last Name

DIAB

MiddleName

-

Affiliation

Department of Animal and Poultry Health and Environment, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.

Email

-

City

-

Orcid

-

First Name

AHMED SHABAN

Last Name

AHMED

MiddleName

-

Affiliation

Department of Food Hygiene and Control (Milk Hygiene), Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.

Email

ahmed_shaaban@vet.svu.edu.eg

City

Qena

Orcid

0000-0003-3794-8295

First Name

LUAY

Last Name

ALKAZMI

MiddleName

-

Affiliation

Biology Department, Faculty of Applied Sciences, Umm Al-Qura University, Makkah 21955, Saudi Arabia.

Email

-

City

-

Orcid

-

First Name

GABER EL-SABER

Last Name

BATIHA

MiddleName

-

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt.

Email

-

City

-

Orcid

-

First Name

MONA A.

Last Name

EL-ZAMKAN

MiddleName

-

Affiliation

Department of Food Hygiene and Control (Milk Hygiene), Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.

Email

-

City

-

Orcid

-

Volume

67

Article Issue

171

Related Issue

25733

Issue Date

2021-10-01

Receive Date

2021-10-03

Publish Date

2021-10-01

Page Start

75

Page End

102

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 67_Issue 171_Pages 75-102.pdf

PDF . 1.4MB

Link

https://avmj.journals.ekb.eg/article_205248.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=205248

Order

8

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

ANTIFUNGAL DISINFECTANTS EFFICIENCY ON ASPERGILLUS STRAINS FROM CAMEL’S MILK AND DRINKING WATER: BIOLOGICAL DETOXIFICATION OF AFLATOXIN-M1

Details

Type

Article

Created At

23 Jan 2023