Due to financial losses associated with avian salmonellosis, high costs of prohibiting its spread and prevention and its multiple drug resistance, Salmonella infection, particularly in ducks, attracted interest of many researchers as duck is a main reservoir of salmonella transmitted to human. Therefore, this study aimed at identifying circulating salmonella of ducks in Assiut governorate and assessing their antimicrobial susceptibility profile. Five hundred and sixty samples (150 livers and 410 cloacal swabs) of infected, freshly dead and apparently healthy ducks, were obtained from different farms in Assiut governorate for bacteriological, serological and molecular examination. An overall 16.6% Salmonella detection rate was recorded, where 15isolates were identified serologically and molecularly as S. typhimurium (93.3%) and S. infantis (6.7%). Basing on antibiogram guidelines, detected Salmonella isolates were completely resistant (100%) to cephradine and amoxicillin, but had variable resistance degrees to colistin sulfate (80%), streptomycin (60%), chloramphenicol (33.3%), ampicillin and neomycin (26.7% of each). MIC test presented that all isolates were absolutely sensitive to colistin and doxycycline, but completely resistant to sulfaquinoxaline. High resistance rates occurred to cephradine, amoxicillin, streptomycin and florfenicol. sul-1, strA-strB, bla TEM, aadA and floR antibacterial resistance genes were assigned in variable frequencies (100%, 73.3%, 73.3%, 66.7% and 46.7%, respectively). In conclusion, S. typhimurium and S. infantis serovars are circulating among duck farms in Assiut. These serotypes exhibited genetic multiple drug resistance, that require special strict biosecurity and searching alternative effective control strategies.