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Isolation and Characterization of some Enterobacteriaceae Isolated from Early Mortalities in Japanese Quail Chicks at Qena Governorate, Egypt

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Microbiology, mycology and immunology

Abstract

Quail farms become widely spread in Egypt as a source of meat. Early mortality of quail
chicks is one of quail breeding limiting factors. This study was performed to study role of
some Enterobacteriaceae as a parable cause of early mortality in South Valley University
quail farm, characterize isolates phenotypically and serologically, determine their
antimicrobial sensitivity and to screen all isolates for presence of florfenicol resistance gene
(floR), Quaternary ammonium compounds resistance gene (QacA/B) and class 1 integrons
gene (Int1) and to screen E. coli, Salmonella and K. pneumoniae isolates for intimin encoding
gene (eaeA), invasive encoding gene (invA) and mucoviscosity associated gene (magA)
respectively. Therefore, 80 freshly dead or moribund Japanese quail chicks under 12 days of
age were collected from this farm during the period from December 2020 to April 2021 for
clinical assessment and bacterial isolation. Totally, 14 E. coli, 6 Salmonella and 1 K.
pneumoniae isolates were isolated and phenotypically identified from the internal organs of
the examined quail chicks with percentage of (17.5%), (7.5%) and (1.25%) respectively. E.
coli isolates belonged to 5 different O-serogroups comprising O86 (28.6%), O78 (21.4%), O26
(14.3%), O55 (14.3%) and O164 (7.1%) in addition to 2 nontypeable isolates (14.3%) while all
Salmonella isolates were serologically identified as S. Typhimurium with antigenic formula
1,4,[5],12:i:1,2. All isolates were weak biofilm producer except (33.3%) of S. Typhimurium
isolates and showed Congo red binding activity except (28.6%) of E. coli isolates while only
(14.3%) of E. coli isolates had hemolytic activity. Antimicrobial susceptibility testing for the
isolates to 10 different antibiotics revealed that all the isolates were sensitive only to
chloramphenicol and oxytetracycline except (42.9%) and (85.7%) of E. coli isolates
respectively and to azithromycin except (35.7%) of E. coli isolates and K. pneumoniae. PCR
revealed that all the isolates harbor Int1 and floR genes, (100%) and (33.3%) of S.
Typhimurium isolates harbor invA and QacA/B genes respectively and only (21.4%) of E. coli
isolates harbor eaeA and QacA/B genes. It was concluded that E. coli and S. Typhimurium are
a major cause of early mortality of Japanese quail chicks.





DOI

10.21608/avmj.2021.89808.1007

Keywords

Enterobacteriaceae, mortalities, Japanese quail, Qena Governorate, Egypt

Authors

First Name

Waleed

Last Name

Younis

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt

Email

walid-younas@vet.svu.edu.eg

City

Qena

Orcid

0000-0001-5572-0142

First Name

Mahmoud

Last Name

Sabra

MiddleName

Hamed

Affiliation

Department of Poultry Diseases, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt

Email

m_sabra@vet.svu.edu.eg

City

Qena

Orcid

0000-0001-6703-2187

First Name

Ehab Kotb

Last Name

Elmahallawy

MiddleName

-

Affiliation

Department of Zoonoses, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt.

Email

eehaa@unileon.es

City

Sohag

Orcid

0000-0003-4484-3678

First Name

Haitham Helmy

Last Name

Sayed

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt.

Email

vet_haitham@yahoo.com

City

-

Orcid

0000-0001-9528-9756

Volume

67

Article Issue

170

Related Issue

25732

Issue Date

2021-07-01

Receive Date

2021-08-02

Publish Date

2021-07-01

Page Start

19

Page End

36

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 67_Issue 170_Pages 19-36.pdf

PDF . 837.8kB

Link

https://avmj.journals.ekb.eg/article_196753.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=196753

Order

3

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

Isolation and Characterization of some Enterobacteriaceae Isolated from Early Mortalities in Japanese Quail Chicks at Qena Governorate, Egypt

Details

Type

Article

Created At

23 Jan 2023