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188816

EFFECT OF MIXTURE OF SODIUM CHLORIDE AND POTASSIUM LACTATE ON THE VIABILITY OF TOXOPLASMA GONDII IN MEAT

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Last updated: 23 Jan 2023

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Abstract

ABSTRACT   The present study is a trial to implement an accurate and easily detectable test applied on meat juice to detect T. gondii. Besides realization the ability of table salt mixture to deactivate T. gondii. In the present study seroprevalence of T. gondii in sheep and cattle meat juice by LAT was 21.43% and emphasis by PCR was 17.14% which indicate very strong agreement between LAT and PCR as a reference test (Kappa = 0.862) with high specificity (94.83%) of meat juice LAT in comparison with PCR and strong relationship between these two tests (Correlation coefficient r = 0.871, p < 0.0001). Sheep meat showed a high prevalence of T. gondii by both LAT and PCR tests (42.86%, 34.29%), respectively. No infection was recorded among the examined cattle meat. There was a very high statistical significance between the prevalence of infection among sheep and cattle by both tests. Both ewes and rams showed the same prevalence of T. gondii 42.9% without showing statistical significance according to the sex of the meat animal. By mice bioassay for salt mixture on the viability of T. gondii, a mixture of (2% sodium chloride and 1.4% potassium lactate) was able to deactivate of T. gondii cyst in sheep meat after 8 hours from exposure, while low concentration (1% of both mixture components) has no effect. 

DOI

10.21608/avmj.2021.188816

Keywords

Keywords: Toxoplasma gondii latex agglutination PCR Sodium chloride Potassium lactate

Authors

First Name

BASEM

Last Name

REFAT NAGEIB

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Affiliation

Department of Parasitology, Animal Health Research Institute, Assiut Regional Lab. (AHRI), Agriculture Research Center, Egypt

Email

basemnageib@gmail.com

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First Name

MOHAMED

Last Name

HAMDY MOHAMED

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Affiliation

Department of Food Hygiene, Animal Health Research Institute, Assiut Regional Lab. (AHRI), Agriculture Research Center, Egypt.

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Volume

67

Article Issue

169

Related Issue

25731

Issue Date

2021-04-01

Receive Date

2021-03-28

Publish Date

2021-04-28

Page Start

37

Page End

53

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_188816.html

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https://avmj.journals.ekb.eg/service?article_code=188816

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4

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Journal

Publication Title

Assiut Veterinary Medical Journal

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https://avmj.journals.ekb.eg/

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Article

Created At

23 Jan 2023