ABSTRACT
 
E. coli is a Gram negative bacterium, although it is normal intestinal inhabitant but some strains due to their virulence genes play a major role in causing diarrhea in birds. In the present study, a total of 150 fecal   swabs from (crows, egret wild   birds and broiler chicken) collected from Giza, Fayoum city governorates, (50 each). The result showed, that  isolation rate of E.coli was reported to be isolated in higher incidence inFayoum governorate from crows, 48%, broiler chicken, 40% and egret wild birds 28% while Giza Governorate in where E.coli was isolated from crows, egret wild birds and broiler chicken ,an incidence of 20%, 12% and 20% respectively. The serogroups of E. coli strains that obtained by serological identification were from crows (O78, O91, O145, O127, O158, O119, O125 and O55), egret bird(O78, O158, O125, O119, O91 and O44) and chicken broiler (O78, O125 and O158). The results of sensitivity test for some E. coli isolates  showed that they were highly resistant for to streptomycin (83.4%66.4%and 42.8%) in (crows, egret birds and broiler chicken) respectively. The results of multiplex PCR showed that phoA,virulence gene was detected in all E.coliserogroups while, Stx2, gene was detected in serogroups O78, O91 and O125 in crows only. (hly, eaeA and Stx1) virulence genes were not detected in all tested  E. colisergroups. On the other hand aadA1 gene was detected by some E. colistrains (7from crows and 2 from egret).