Bovine coronavirus (BCoV) infection has found worldwide among cattle of all ages, causing diarrhea in newborn calves, winter dysentery in adult cattle and respiratory tract diseases results in major economic losses in dairy herds. The objective of this study was to establish an ELISA kits as patent prepared in Lab. with assessment its applicability in the field samples. Sixty-five pneumonic calves 2- to 4-month-old, used in this study to detect BCoV antibodies in serum of infected calves, where it assayed against BCoV antibodies by using prepared ELISA plates and SNT. The results were positive for BCoV antibody 72.3% and 53.9% by ELISA and SNT respectively. The prepared ELISA showed high sensitivity of over SNT, where sensitivity, specificity and correlation were 91.4%, 50% and 72.3% respectively, and there is a positive correlation between both tests. The test has been successfully used in longitudinal field studies on virus dynamics and evaluation of management routines. In conclusion the patent prepared ELISA is more sensitive, accurate and specific assay than SNT for detection of BCoV antibodies, in addition to saving time and commercial kits cost.