In this study we investigate theeffectiveness of intranasal vaccination of calves with live streptomycin-dependent mutant of P.multocida type B vaccine in comparison with inactivated one. Live P.multocida vaccine gave higher mitogenic response of the peripheral blood lymphocytes as measured by lymphocyte blastogenesis assay than inactivated one allover the period of the experiment. The haemagglutinating antibodies as measured by Passive haemagglutination test (HA) and the IgG as measured by ELISA rose from one day to reach the peak at 15 days, and then decline till 12 weeks post vaccination for live vaccine. However for inactivated vaccine it reached the peak at 8 weeks and then decline till 12 week post vaccination. Calves with live P.multocida vaccine gave higher and earlier secretory IgA in nasal secretions than inactivated one. In conclusion, the intranasal administration of live streptomycin-dependent serotype of P.multocida mutant vaccine was efficient and induced local cellular immune response, IgA antibodies and systemic IgG antibodies.