A total of 85 freshly dead (growing quails 7-40 days) collected from different farms of kena province. The clinical signs of the infected quails were sever weakness, depression, retardation of growth as well as whitish diarrhoea, while the most postmortem lesions were sever congestion of the paranchymatous organs and unabsorbed yolk sac. Isolation revealed 29 isolates of positive cases. The isolates were as follows, 10 E. coli, 7 S. pullorurm, 5 Staph. aureus, 4 Staph. epidermidis, 2 S.typhimurium and1 B. cereus respectively with an incidence of 11.8%, 8.2%, 5.9%, 4.7%, 2.4% and 1.2%. Trails for reproducing the infection in 3 day old quails lead to 70% mortality. The clinical observation and postmortem lesions were similar to great extent to those of natural infection. Reisolation of the inoculated organism from dead quails were conducted. In vitro antibiotics sensitivity test showed that the examined of E.coli isolates were highly sensitive to enrofloxacin, danofloxacin, flumequin and gentamycin while enrofloxacin, danofloxacin, flumequin, gentamycin, lincomycin and chloramphenicol against Salmonella isolates.