This work aimed to study the effect of  physico-chemical agents  on the stability and biological properties of local strain of goat pox virus (GPV) as well as perform haemagglutination test and cross neutralization test between this virus and sheep pox virus. The virus was propagated on VERO cells and produce CPE specific to pox virus. On study the effect of heat on the virus, the results showed that GPV was stable at both room temperature and 37ºC for 7 successive days while the virus reduced its infectivity after 2 hours at 56ºC; the virus was stable at 4 repeating cycles of freezing and thawing. On studying the effect of ultra violet rays UVR the result showed that the virus was sensitive to UVR. Regarding to the effect of chemical agents the results revealed that; the effect of 0.5% formalin on the virus infectivity  resulted in decreased virus infectivity gradually from 10⁵ to 10^0.5 TCID₅₀ /ml over 24 hours of treatment; the effect of 20% ethyl ether on the virus infectivity the result showed that virus infectivity decreased gradually till reach 10² TCID _5O  /ml. over 24 hours of treatment; the effect of both 50% alcohol and 0.01% potassium permanganate on the infectivity, revealed that virus was inactivated within one hour of treatment at room temperature. Regarding to cross neutralization test; there was a cross neutralization between goat pox and sheep pox virus which concluded that there was antigenic relationship between the two viruses. The result of Haemagglutination test revealed that the isolated virus agglutinate RBCs of chickens.