The objective of this study was to evaluate the effects of different trials for treatment of post partum (p.p.) ovarian inactivity in buffaloes and their economic values. 75 buffalo-cows not observed in estrus 3 months followed parturition, were used in this study. Clinical examinations and progesterone assay revealed ovarian inactivity. The animals were randomly allocated to 5 treatment groups. Group I (GI) was kept as control (injected by 5 ml saline). Group II (GII) was injected by 0.02 mg buserelin (5 ml Receptal, GnRH analogue). Group III (GIII) was injected by 40mg progesterone for successive 8 days and 0.02 mg buserelin at 8th day. Group IV (GIV) was injected by 40mg progesterone for successive days and 2mg and lmg estradiol benzoate at 1st and 8th day respectively. Group V (GV) was injected by 10 ml tonophosphane daily for 2 days and received 200 gm disodium phosphate and 250 gm mineral mixture divided into 5 days with bran mash. All buffalo-cows were observed for estrus detection three times daily (at dawn, afternoon and evening). Animals came in estrus were naturally mated using fertile buffalo-bulls and their plasma were tested for progesterone level 8 days after mating. While animals did not came in estrus were weekly examined. Pregnancy diagnosis was done using rectal palpation 42 days after mating. The results indicated that, buffalo-cows came in estrus were 40%, 73.3%, 80%, 60% and 53.3% in GI, GII, GIII, GIV and GV respectively. GIII showed the shortest treatment-estrus interval (TEI), treatment conception interval (TCI) and days open (DO) (8.1+1.7, 20.9=4.7 and 116.4+6.1 days, respectively), followed by GIV (18.3+5.7, 31.9+5.7 and 127.76.1 days, respectively). While, the longest TEI, TCI and DO (52.6+4.6, 66.5+6.2 and 161.636.9 days, respectively) was obtained in GI. The lowest service per conception (S/C) (1.60+0.2) was obtained in GIII while, the highest was obtained in GV (1.86+0.3). The highest 1st service conception rate was obtained in GIII (60%) while, it was equal in other groups (46.7%). Plasma progesterone assay revealed 215 Assiut Vet. Med. J. Vol. 52 No. 111 October 2006 highly significant increase in p < span style="text-decoration: underline;">rogesterone levels at day 8 post service >ing /ml) indicating functioning corpora lutea. The obtained net profit, profit/cost ratio, net profit/return and net profit/cost in GIII were the highest followed by that of GIV. In conclusion, hormonal treatment of buffalo-cows proved useful in reducing the calving interval and to increase fertility.