Present study was preformed on 90 frozen samples of meat, poultry and fish (30 of each).  The samples were collected from different shops. in Assiut city . Listeria  species were isolated at different percentages (33%) in frozen meat, (60%) in frozen poultry and (53%) in frozen fish. Also they were counted in the examined samples, the mean counts were 2.9×10³±1.9, 5.65×10² ± 4.7 CFU/g and 12.75×10² ± 6.8 CFU/g of the examined frozen samples of meat, poultry and fish respectively. Listeria  monocytogenes was differentially identified from other Listeria  species and could be isolated at variable percentages (16.6%) in frozen meat, (33%) in frozen poultry and (13.3%) in frozen fish, the study revealed that the incidence of L.monocytogenes was higher in frozen poultry as compared to both frozen meat and fish. The drug susceptibility characterization of L.maonocytogenes cleared that all isolates were resistant (100%) to Gentamicin and Ampicillin but were susceptible (100%) to chloramphenicol and Norfloxacin, while the other used antibiotic show different degree of antimicrobial sensitivity reactions for streptomycin, Tetracycline, Rifampin, Cefadroxil and Cefotaxime.